Cell expansion genes expression by therapeutic ultrasound. pros and cons.
Keywords:Acids, Acoustic waves, Bone, Bottles, Cells, Colloids, Cytology, Gelation, Gene expression, Ketones, Organic acids, Plants (botany), Ultrasonics, Agarose gels, Ascorbic acids, Basic medias, Bone marrow cells, Bone marrows, Cell expansions, Culture mediums, Duty-cycle, Fetal Bovine serums, Genes expressions, Low intensities, Pcr reactions, Pulsed ultrasounds, Self renewals, Stem cells, Therapeutic ultrasounds
AbstractLow intensity pulsed ultrasound (LIPUS) could up-regulate Nucleostemin gene in rapidly proliferative and self renewal cells, was hypothesized. The bone marrow cells (BMSC) were isolated from tibiae and femora of 3 month old male Sprague-Dawley rats and the bone marrow was flushed with basic media using 10-ml syringe from both sides. Cells were seeded in in 75 mm flasks and were expanded in culture medium that contained 10% fetal bovine serum (FBS), 1% streptomycin, and ascorbic acid. LIPUS was applied for 20 minutes per day to the base of the cell culture flasks using a 2.5 cm2 transducer that delivers 1.5 MHz with a duty cycle of a quarter. Samples were amplified for 22-30 cycles in an Eppendrof Master-cycle thermal cycler, and PCR reactions were electrophoresed on 2% agarose gels. The results show that LIPUS has increased Nucleostemin expression in both types of stem cells.
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