Low intensity pulsed ultrasound stimulates osteogenic differentiation of human gingival fibroblasts
Keywords:Acoustic waves, Cell culture, Cytology, Differentiation (calculus), Fibroblasts, Gene expression, Nucleic acids, Organic acids, Ultrasonics, Alp activities, Bone cells, Cell activities, Cell viabilities, DNA contents, Human gingival fibroblasts, Low intensities, Osteogenic differentiations, Pulsed ultrasounds, Well plates
AbstractA study was conducted to investigate the effect of low intensity pulsed ultrasound (LIPUS) on osteogenic differentiation of human gingival fibroblasts (HGF). Bone cells, cementoblasts, and odontoblast cells are very sensitive to LIPUS exposure. The study used HGF cells cultured in 48-well plates at 2.5×103 cells/well and analyzed different cell activities. The study used MTT (4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays to measure the cell viability. The study observed an increase in total DNA content and LIPUS treatment can regulate OPN gene expression. The study also found that the LIPUS stimulation can enhance the differentiation of HGF by increasing ALP activity and OPN expression. The LIPUS stimulation can be used for therapeutic treatment of odotoblast cells.
How to Cite
Copyright on articles is held by the author(s). The corresponding author has the right to grant on behalf of all authors and does grant on behalf of all authors, a worldwide exclusive licence (or non-exclusive license for government employees) to the Publishers and its licensees in perpetuity, in all forms, formats and media (whether known now or created in the future)
i) to publish, reproduce, distribute, display and store the Contribution;
ii) to translate the Contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or, abstracts of the Contribution;
iii) to exploit all subsidiary rights in the Contribution,
iv) to provide the inclusion of electronic links from the Contribution to third party material where-ever it may be located;
v) to licence any third party to do any or all of the above.