Low intensity pulsed ultrasound stimulates osteogenic differentiation of human gingival fibroblasts

Authors

  • Nesrine Mostafa Departments of Dentistry and Biomedical Engineering, University of Alberta, Edmonton, AB, Canada
  • Paul Scott Department of Biochemistry, University of Alberta, Edmonton, AB, Canada
  • Douglas N. Dederich Department of Dentistry, University of Alberta, Edmonton, AB, Canada
  • Michael Doschak Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
  • Tarek El-Bialy Departments of Dentistry and Biomedical Engineering, University of Alberta, Edmonton, AB, Canada

Keywords:

Acoustic waves, Cell culture, Cytology, Differentiation (calculus), Fibroblasts, Gene expression, Nucleic acids, Organic acids, Ultrasonics, Alp activities, Bone cells, Cell activities, Cell viabilities, DNA contents, Human gingival fibroblasts, Low intensities, Osteogenic differentiations, Pulsed ultrasounds, Well plates

Abstract

A study was conducted to investigate the effect of low intensity pulsed ultrasound (LIPUS) on osteogenic differentiation of human gingival fibroblasts (HGF). Bone cells, cementoblasts, and odontoblast cells are very sensitive to LIPUS exposure. The study used HGF cells cultured in 48-well plates at 2.5×103 cells/well and analyzed different cell activities. The study used MTT (4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays to measure the cell viability. The study observed an increase in total DNA content and LIPUS treatment can regulate OPN gene expression. The study also found that the LIPUS stimulation can enhance the differentiation of HGF by increasing ALP activity and OPN expression. The LIPUS stimulation can be used for therapeutic treatment of odotoblast cells.

Published

2008-09-01

How to Cite

1.
Mostafa N, Scott P, Dederich DN, Doschak M, El-Bialy T. Low intensity pulsed ultrasound stimulates osteogenic differentiation of human gingival fibroblasts. Canadian Acoustics [Internet]. 2008Sep.1 [cited 2021Apr.13];36(3):34-5. Available from: https://jcaa.caa-aca.ca/index.php/jcaa/article/view/2024

Issue

Section

Proceedings of the Acoustics Week in Canada