Low intensity pulsed ultrasound stimulates osteogenic differentiation of human gingival fibroblasts
Keywords:
Acoustic waves, Cell culture, Cytology, Differentiation (calculus), Fibroblasts, Gene expression, Nucleic acids, Organic acids, Ultrasonics, Alp activities, Bone cells, Cell activities, Cell viabilities, DNA contents, Human gingival fibroblasts, Low intensities, Osteogenic differentiations, Pulsed ultrasounds, Well platesAbstract
A study was conducted to investigate the effect of low intensity pulsed ultrasound (LIPUS) on osteogenic differentiation of human gingival fibroblasts (HGF). Bone cells, cementoblasts, and odontoblast cells are very sensitive to LIPUS exposure. The study used HGF cells cultured in 48-well plates at 2.5×103 cells/well and analyzed different cell activities. The study used MTT (4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays to measure the cell viability. The study observed an increase in total DNA content and LIPUS treatment can regulate OPN gene expression. The study also found that the LIPUS stimulation can enhance the differentiation of HGF by increasing ALP activity and OPN expression. The LIPUS stimulation can be used for therapeutic treatment of odotoblast cells.Additional Files
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